HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Research Technical Services

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Research Technical Services

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

High performance liquid chromatography is one of the most widely used separation techniques due to its versatility and wide field of application. The components of the sample are forced to pass through the chromatographic column where they are separated from one another. The process involves various chemical and/or physical interactions between the molecules of the sample and the packing particles of the column. The resolution of this separation depends on the interaction between the stationary phase and the mobile phase, and can be optimized by selecting the appropriate solvent mixtures and fillers of the column. As a final result, the components of the mixture leave the column separated according to their retention times leading to the chromatogram. Qualitative and quantitative identification of the separated species can be performed using the different detectors available

 

  

Applications

 

 

Equipment

 

  • Agilent's uHPLC 1260 Infinity Binary LC System:
    • Automatic injection
    • Binary pump
    • Thermostatted column compartment with 2 selectable channels.
    • Thermostatted sample compartment.
    • Detection systems:
      • DAD (Diode Array Detector)
      • FLD (Fluorescence Detector)
      • RID (Refractive Index Detector)

 

 

Sample requirements